HPLC Solvents Product Description
High-Performance Liquid Chromatography (HPLC) solvents are essential for the separation, identification, and quantification of compounds in various samples. These solvents are specifically formulated to provide optimal performance in HPLC systems, ensuring high resolution, reproducibility, and sensitivity in analytical results. Commonly used HPLC solvents include water, acetonitrile, methanol, and various buffers, each serving distinct purposes in the chromatographic process.
Uses of HPLC Solvents
- Mobile Phase Composition: HPLC solvents are used to create the mobile phase, which carries the sample through the chromatographic column.
- Sample Preparation: They are often employed in the preparation of samples to ensure that analytes are in a suitable form for analysis.
- Gradient Elution: Different solvents can be mixed to create a gradient, allowing for the separation of compounds with varying polarities.
- Cleaning and Maintenance: HPLC solvents are also used for cleaning the system and maintaining the integrity of the equipment.
Technical Data
| Property | Water | Acetonitrile | Methanol | Buffer Solutions |
|---|---|---|---|---|
| Purity | ≥ 99.9% | ≥ 99.9% | ≥ 99.9% | Varies (typically ≥ 99%) |
| Polarity | High | Moderate | Moderate | Varies |
| Viscosity (cP) | 0.89 | 0.36 | 0.54 | Varies |
| Boiling Point (°C) | 100 | 82 | 65 | Varies |
| pH Range | Neutral (7) | Neutral (7) | Neutral (7) | Varies (depends on buffer) |
| Applications | General use, aqueous samples | Organic compounds, pharmaceuticals | Polar compounds, biological samples | pH control, ion pairing |
| Storage Conditions | Cool, dark place | Cool, dark place | Cool, dark place | As per buffer specifications |
Key Features
- High Purity: Ensures minimal interference and background noise in analytical results.
- Compatibility: Suitable for a wide range of HPLC applications, including reverse phase, normal phase, and ion-exchange chromatography.
- Versatility: Can be used in various combinations to optimize separation conditions based on the specific requirements of the analysis.
In summary, HPLC solvents are critical components in the field of analytical chemistry, providing the necessary properties for effective separation and analysis of complex mixtures. Their selection and use are pivotal in achieving accurate and reliable results in various applications, including pharmaceuticals, environmental testing, and food safety.