HPLC Solvents Product Description
High-Performance Liquid Chromatography (HPLC) solvents are essential for the separation, identification, and quantification of compounds in various samples. These solvents are characterized by their high purity, low viscosity, and ability to dissolve a wide range of analytes. They are used in various applications, including pharmaceuticals, environmental testing, food and beverage analysis, and biochemical research. The choice of solvent can significantly affect the efficiency and resolution of the chromatographic process, making it crucial to select the appropriate solvent for specific applications.
Uses of HPLC Solvents
- Pharmaceutical Analysis: Used for the separation and quantification of active pharmaceutical ingredients (APIs) and impurities.
- Environmental Testing: Employed in the analysis of pollutants and contaminants in water, soil, and air samples.
- Food and Beverage Testing: Utilized for the detection of additives, preservatives, and contaminants in food products.
- Biochemical Research: Important for the purification and analysis of proteins, nucleic acids, and other biomolecules.
- Quality Control: Essential in laboratories for routine testing and quality assurance of products.
Technical Data Details
| Property | Description |
|---|---|
| Purity | ≥ 99.9% (HPLC grade) |
| Viscosity | Low viscosity for optimal flow rates |
| pH Range | Neutral (typically 6-8) |
| Boiling Point | Varies by solvent (e.g., Acetonitrile: 82 °C) |
| Density | Varies by solvent (e.g., Methanol: 0.791 g/cm³) |
| Solubility | Miscible with water and other organic solvents |
| Storage Conditions | Store in a cool, dry place, away from light |
| Shelf Life | Typically 2 years when stored properly |
Common HPLC Solvents
- Acetonitrile: Widely used for its low viscosity and high elution strength.
- Methanol: Commonly used due to its excellent solvent properties and low cost.
- Water: Often used as a mobile phase component, especially in reversed-phase HPLC.
- Tetrahydrofuran (THF): Used for its ability to dissolve a wide range of organic compounds.
- Dimethyl sulfoxide (DMSO): Known for its high polarity and ability to dissolve polar and nonpolar compounds.
Conclusion
Selecting the right HPLC solvent is critical for achieving optimal separation and analysis results. The purity, viscosity, and compatibility of the solvent with the analytes and the chromatographic system must be considered to ensure accurate and reproducible results.